5 Simple Statements About flow cytometers Explained
5 Simple Statements About flow cytometers Explained
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Data-enriched characterization of mobile populations. Morphological information and facts from illustrations or photos can incorporate on the richness of flow cytometry facts. By way of example, the determine exhibits an or else typical apoptosis assay making use of Annexin V and PI, introducing cell imaging to characterize cells in each population to reveal morphologically unique characteristics. These insights could not are acquired from multiplex staining by yourself.
As samples are acquired around the Attune CytPix Flow Cytometer, the substantial-speed brightfield digital camera captures and stores photographs of detected functions at a fee nearly six,000 images for every second, based on the flow price and picture size. For higher flexibility, Attune Cytometric Application enables you to modify the graphic capture frequency as wanted and choose a specific gate for graphic seize, if necessary.
Incorporate acoustic concentrating technologies with higher-parameter analysis for adaptable spectral unmixing and conventional workflows.
Find out new insights into your experimental data by visualizing populations using put together fluorescence and morphology data. Our computer software interface wizards allow downsampling, concatenation (merged files) and dimensionality equipment that happen to be developed in to the application for an intuitive, streamlined knowledge Assessment experience.
"A picture is not pretty value 1000 dots, but it surely actually helps to see illustrations or photos. It is great to see images that correlate with staining. As an example, AnnexinV-beneficial cells which have been morphologically various than healthy cells, or CD14+ monocytes that happen to be larger plus much more textured than CD3+/CD4+/CD14- lymphocytes.
Here, an experienced person has gated singlets confidently. Soon after analyzing the guide singlet gate, the CytPix image-derived parameter ParticleCount reveals this gate has in excess of 4% aggregates.
Optimizing gating strategy. Even sturdy handbook singlet gating is mistake-prone and remains a subjective choice place in Nearly all flow cytometry assays. Imaging can be utilized to verify and alter gates to include only single cells of interest.
Our experts will style panels for you here personally as over and over you require—totally free, no product purchase required.
Early signs of this contamination experienced to begin with been dismissed as debris. Backgating to discover new populations. Backgating imaged cells on the Attune CytPix Flow Cytometer also helps you to use morphological capabilities to find intriguing subpopulations that would not be clear from flow cytometry data by yourself.
Buyers can watch celebration photographs in genuine-time to verify sample excellent, enhance protocols, and history the morphology of each and every mobile inhabitants discovered for Investigation. Imaging seize also Added benefits from acoustic focusing, which really helps to place the cells so that a pointy, centered image is received.
AI-driven automatic picture Investigation Along with the Attune Cytometric Application translates occasion attributes into unique morphology parameters that may be coupled with common fluorescence and scatter parameters to expose remarkably reproducible population changes and unique subsets.
We Beforehand demonstrated the power of imaging Auto-T/Ramos mobile interactions. Permit’s take a look at just the populace of best desire, the double favourable situations, to learn more. We could now use prolonged morphological parameters (listed here, circularity and skewness of depth) to additional look at the functions of these populations and refine gating on these gatherings, expanding details robustness.
Leverage the strength of Superior engineering and innovation to speed up your discoveries. Have confidence in from the Attune Flow Cytometer family to deliver the reliability, pace, and precision that established your do the job apart.
No wash/no lyse leukocyte Evaluation with imaging. Cells had been acquired from 24-hour-old blood by dilution in 1 mM EDTA ( Pro UX/UI permits consumers to teach AI types for expanded purposes. (A) The “Coach Design” function offers annotation tools and actual-time model schooling precision.
At the center of each and every Attune Flow Cytometer lies our innovative acoustic focusing technologies, which makes use of seem waves to meticulously align cells within the flow mobile. This revolutionary solution surpasses classic hydrodynamic procedures, resulting in a lot quicker processing speeds, exceptional data good quality, and improved sample throughput.
Because the cell line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went again to the pictures and ended up in a position to doc the microbial an infection at the least 5 days previously.
Agilent Worth Guarantee ensures you no less than a decade of instrument use through the day you buy. Usually, we will credit score you While using the residual value of the technique towards an upgraded model.
What gave the impression to be a homogeneous population is really more assorted—an insight that needs to be thought of when interpreting outcomes.